- PII
- S30345057S2686738925030058-1
- DOI
- 10.7868/S3034505725030058
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 522 / Issue number 1
- Pages
- 345-350
- Abstract
- The method of intravital bioimaging based on luminescence occupies an important place in the development and testing of antitumor drugs on model animals and is an essential part of preclinical studies. Bioimaging based on luminescent systems, compared with fluorescent bioimaging, provides a high signal-to-noise ratio, which justifies the development of cell lines, that stably express luciferase genes for subsequent use in model animals. The work describes the creation of a stable cell line SKOV3.ip1-NanoLuc constitutively expressing the NanoLuc luciferase gene. The developed cell line was shown to be effective for intravital luminescence bioimaging of immunodeficient animals with deep-seated intraperitoneal tumors, which can be considered as a model of late-stage ovarian cancer.
- Keywords
- люцифераза NanoLuc аденокарцинома рака яичника стабильная клеточная линия биоимиджинг
- Date of publication
- 15.04.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 44
References
- 1. Tuguntaev R.G., Hussain A., Fu C., et al. Bioimaging guided pharmaceutical evaluations of nanomedicines for clinical translations // J Nanobiotechnol. 2022. Vol. 20. P. 236.
- 2. Yu D., Wolf J.K., Scanlon M., et al. Enhanced c-erbB-2/neu expression in human ovarian cancer cells correlates with more severe malignancy that can be suppressed by E1A // Cancer Res. 1993. Vol. 53, №4. P. 891-898.
- 3. Galogre M., Rodin D., Pyatnitskiy M., et al. A review of HER2 overexpression and somatic mutations in cancers // Critical Reviews in Oncology/Hematology. 2023. Vol. 186. P. 103997.
- 4. Hall M.P., Unch J., Binkowski B.F., et al. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate // ACS Chem Biol. 2012. Vol. 7. №11 P. 1848-57.
- 5. Shramova E.I., Chumakov S.P., Shipunova, V.O., et al. Genetically encoded BRET-activated photodynamic therapy for the treatment of deep-seated tumors // Light Sci Appl. 2022. Vol. 11. P. 38.
- 6. Deyev S., Proshkina G., Baryshnikova O., et al. Selective staining and eradication of cancer cells by proteincarrying DARPin-functionalized liposomes // European Journal of Pharmaceutics and Biopharmaceutics. 2018. Vol. 130. P. 296-305.
- 7. Taylor A., Sharkey J., Plagge A., et al. Multicolour in vivo bioluminescence imaging using a NanoLucbased BRET reporter in combination with firefly luciferase // Contrast Media & Molecular Imaging. 2018. P. 2514796.
- 8. Ogawa M., Takakura H. Optical-based detection in live animals. In: Tanaka K., Vong K., editors. Handbook of in vivo chemistry in mice. Wiley-VCH Verlag GmbH & Co. KgaA. 2020. P. 55-101.
- 9. Sokolova E.A., Shilova O.N., Kiseleva D.V., et al. HER2-specific targeted toxin DARPin-LoPE: immunogenicity and antitumor effect on intraperitoneal ovarian cancer xenograft model // Int. J. Mol. Sci. 2019. Vol. 20. № 10. P. 2399.
