- PII
- S30345057S2686738925020089-1
- DOI
- 10.7868/S3034505725020089
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 521 / Issue number 1
- Pages
- 214-218
- Abstract
- Previously, polypeptide constructs - modular nanotransporters (MNT) - were created to deliver biologically active molecules into the nuclei of melanoma cells. In the present work, polyethylene glycol (PEG) molecules were attached to them at the N-terminal cysteine, both with the possibility of their subsequent cleavage at the hydrolysis site of tumor-specific proteases, and without this site (non-detachable PEG). All MNT variants labeled with the radioisotope In were administered to mice with inoculated Cloudman S91 melanoma. The kinetics of radioactivity distribution in the mouse body was studied using single-photon emission computed tomography. Analysis of the obtained data using a compartmental mathematical model allowed us to establish that the attachment of PEG to MNT increased its lifetime in the blood and significantly increased its accumulation in the tumor. Addition of a PEG detachment site by tumor-specific protease led to a strong retention of this MNT in the tumor. The data obtained can serve as a basis for the creation of new effective antitumor drugs.
- Keywords
- модульные нанотранспортеры мелонома полиэтиленгликоль однофотонная эмиссионная компьютерная томография опухолеспецифичные протеазы компартментная модель
- Date of publication
- 15.04.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 56
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