- PII
- S2686738925010062-1
- DOI
- 10.31857/S2686738925010062
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 520 / Issue number 1
- Pages
- 31-37
- Abstract
- Vaccination against brucellosis is currently carried out using attenuated strains of live brucellae, which causes serious side effects in animals and is of little use for human vaccination. In order to develop a safer vaccine against brucellosis, we created plasmid DNA capable of expressing six brucellae proteins in mammalian cells. Linear polyethyleneimine was used to increase the efficiency of DNA delivery to cells. Data on optimization of the conditions for the formation of DNA-polyethyleneimine complexes are presented. It is shown that a single intramuscular or subcutaneous injection of plasmid DNA complexes with polyethyleneimine at a low dose (20 μg/mouse) has a reliable protective effect against subsequent infection of mice with a test strain of brucellae.
- Keywords
- бруцеллез ДНК вакцина полиэтиленимин полицистронная РНК трансфекция in vivo
- Date of publication
- 15.09.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 20
References
- 1. Pappas, G., Papadimitriou, P., Akritidis, N., et al., The new global map of human brucellosis, Lancet Infect. Dis. 2006. Vol. 6, № 2, Р. 91–99.
- 2. Pappas, G., Akritidis, N., Bosilkovski, M., and Tsianos, E., Brucellosis, N. Engl. J. Med. 2005. Vol. 352. № 22, Р. 2325–2336.
- 3. Xie, J., Wang, J., Li, Z., ey al., Ontology-Based Meta-Analysis of Animal and Human Adverse Events Associated With Licensed Brucellosis Vaccines, Front. Pharmacol. Med. 2005. Vol. 352. № 22. P. 503.
- 4. Ivanov, A.V., Salmakov, K.M., Olsen, S.C., Plumb, G.E., A live vaccine from Brucella abortus strain 82 for control of cattle brucellosis in the Russian Federation, Anim. Health. Res. Rev. 2011. Vol. 12, № 1. Р. 113–121.
- 5. Девришов, Д.А., Ельшазли, М.А., Антигенная активность рекомбинантных ДНК B. melitensis Rev-1, Ветеринария, зоотехния и биотехнология. 2019. № 10. С. 7–14.
- 6. Yu, D.H., Hu, X.D., and Cai, H., A combined DNA vaccine encoding BCSP31, SOD, and L7/L12 confers high protection against Brucella abortus 2308 by inducing specific CTL responses, DNA Cell Biol. 2007. Vol. 26, № 6. P. 435–443.
- 7. Casper, J., Schenk, S.H., Parhizkar, E., et al., Polyethylenimine (PEI) in gene therapy: Current status and clinical applications, J. Control. Release. 2023. Vol. 362. Р.667–691.
- 8. Ito, T., Yoshihara, C., Hamada, K., and Koyama, Y., DNA/polyethyleneimine/hyaluronic acid small complex particles and tumor suppression in mice, Biomaterials. 2010. Vol. 31. № 10. Р. 2912–2918.
- 9. Sharma, V.K., Thomas, M., Klibanov, A.M., Mechanistic studies on aggregation of polyethylenimine-DNA complexes and its prevention, Biotechnol. Bioeng. 2005. Vol. 90. № 5. Р. 614–620.
